PHYTOCHEMICAL ANALYSIS AND ANTIMICROBIAL POTENTIAL OF EXTRACTS AND FRACTIONS OF BROSIMUM GAUDICHAUDII TRÉCUL AGAINST BACTERIA OF CLINICAL IMPORTANCE

Autores

  • Antônia Fernanda Lopes da Silva
  • Francisléia Falcão França Santos Siqueira
  • Wenderson Costa da Silva
  • Márcio Leonardo de Morais Nobre
  • Fátima Maria de Souza Pereira
  • Vitor Emanuel Sousa da Silva
  • Francinete Sousa de Oliveira
  • Eliana Campêlo Lago

DOI:

https://doi.org/10.25110/arqsaude.v27i7.2023-007

Palavras-chave:

Antibacterial Potential, Brosimun, Moraceae, Plant Compounds

Resumo

Medicinal plants constitute an arsenal of products with different potentials to be explored. Therefore, the objective of this paper was to assess the antimicrobial potential of the ethanolic extract from Brosimum gaudichaudii leaves and fractions against clinically important bacteria. The crude extracts and fractions from the leaves and stem bark were used against Escherichia coli, Klesbsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus strains. The crude extracts and the fractions were obtained by means of maceration in ethanol and chemically characterized. In the results of the phytochemical screening, the presence of a variety of secondary metabolites was verified, such as flavonoids, steroids, saponins, alkaloids, tannins and coumarins. The extracts and their fractions showed inhibitory activity for all three bacteria tested. The inhibition halo varied from 8±0.00 to 14±0.00 mm fir K. pneumoniae, from 8±0.00 to 10±0.00 mm for P. aeruginosa and from 8±0.00 to 9±0.00 mm for S. aureus. Among the fractions tested, the ethyl acetate fraction from both the stem and the leaves presented the best inhibition potential. This indicates that the Brosimum gaudichaudii Trécul vegetable extracts present antimicrobial potential. Such being the case, it is suggested to isolate the metabolites present in this fraction to delimit the main compounds responsible for the antimicrobial action.

Referências

ABREU, Viviane Gomes da Costa et al. Pentacyclic triterpenes and steroids from the stem bark of uchi (Sacoglottis uchi, Humiriaceae). Acta Amazônica, v. 43, p. 525-528, 2013.

ALMEIDA MEIRA-NETO, R. A.; S. S. Phytochemical, Microbiological and Cytotoxic Evaluation of Leaves of Gossypium arboreum L. (Malvaceae). Biota Amazônia, 5, 18-22. 2015.

ALVES, C. C. F. et al. Antiproliferative activity of essential oils from three plants of the Brazilian Cerrado: Campomanesia adamantium (Myrtaceae), Protium ovatum (Burseraceae) and Cardiopetalum calophyllum (Annonaceae). Brazilian Journal of Biology, v. 80, p. 290-294, 2019.

ARNOUS, Amir Hussein; SANTOS, Antonio Sousa; BEINNER, Rosana Passos Cambraia. Medicinal plants for home use - popular knowledge and interest in community cultivation. Space for Health Magazine, v. 6, no. 2, p. 1-6, 2005.

BAUER AW. Antibiotic susceptibility testing by a standardized single disc method. Am J clin pathol, 45, 149-158, 1966.

BORGES, Joelma da Costa et al. Evaluation of antibacterial activity of the bark and leaf extracts of Brosimum gaudichaudii Trécul against multidrug resistant strains. Natural product research, v. 31, n. 24, p. 2931-2935, 2017.

BRUM, Thiele Faccim et al. HPLC analysis of phenolics compounds and antioxidant capacity of leaves of Vitex megapotamica (Sprengel) Moldenke. Molecules, v. 18, n. 7, p. 8342-8357, 2013.

CAVALCANTE, Giani Maria et al. Antimicrobial activity of Artocarpus heterophyllus Lam. (Moraceae) on the development of Streptococcus pneumoniae and Escherichia coli. Full Scientia, v. 9, no. 2, 2013.

CECHINEL FILHO, Valdir; YUNES, Rosendo A. Estrategies for obtaining pharmacologically active compounds from medicinal plants: concepts about structural modification for improve the activity. Química Nova, v. 21, p. 99-105, 1998.

CHANG, Mau-Sun et al. Furocoumarin Glycosides from the Leaves of Ficus r uficaulis Merr. var. a ntaoensis. Journal of natural products, v. 68, n. 1, p. 11-13, 2005.

COSTA, Anderson Luiz; SOUZA SILVA JUNIOR, Antonio Carlos. Bacterial resistance to antibiotics and public health: a brief literature review. ESTACAO CIENTIFICA-UNIFAP, v. 7, n. 2, p. 45-57, 2017.

EL-SEEDI, Hesham et al. Antimicrobial properties of Apis mellifera’s bee venom. Toxins, v. 12, n. 7, p. 451, 2020.

FRANCO, D. P. et al. A IMPORTÂNCIA DAS CUMARINAS PARA A QUÍMICA MEDICINAL E O DESENVOLVIMENTO DE COMPOSTOS BIOATIVOS NOS ÚLTIMOS ANOS. Química Nova, v. 44, n. 2, p. 180–197, 2021.

GEOGRAPHIC COORDINATE SYSTEMS. Datum: Sirgas. Database: IBGE. Org.: D. L. Made in QGIS, version 3.22, 2000.

GHUMAN, S. et al. Antioxidant, anti-inflammatory and wound healing properties of medicinal plant extracts used to treat wounds and dermatological disorders. South African journal of botany, v. 126, p. 232-240, 2019.

GOBBO-NETO, Leonardo; LOPES, Norberto P. Medicinal plants: factors influencing the content of secondary metabolites. New Chemistry , v. 30, p. 374-381, 2007.

GOULART, EG et al. Ontogenic inhibition of S. stercoralis and Hookworms, in the free-living phase, by natural products. Rev. Bras. of Pharmacy , v. 56, p. 39-47, 1975.

JOAO MATOS, Maria et al. Looking for new targets: simple coumarins as antibacterial agents. Medicinal chemistry, v. 8, n. 6, p. 1140-1145, 2012.

JÜTTE, Robert et al. Herbal medicinal products–Evidence and tradition from a historical perspective. Journal of ethnopharmacology, v. 207, p. 220-225, 2017.

KABERA, Justin N. et al. Plant secondary metabolites: biosynthesis, classification, function and pharmacological properties. J. Pharm. Pharmacol, v. 2, n. 7, p. 377-392, 2014.

KUMAR, Shashank; PANDEY, Abhay K. Chemistry and biological activities of flavonoids: an overview. The scientific world journal, v. 2013, 2013.

LUZ, Raphael F. et al. 13 C-NMR Data from Coumarins from Moraceae Family. American Journal of Analytical Chemistry, v. 6, n. 11, p. 851, 2015.

MALTA LG. Biological evaluation of fruits from the Brazilian Cerrado-Guapeva, Gabiroba and Murici (Doctoral dissertation, State University of Campinas (UNICAMP). Faculty of Food Engineering). 2018.

MATOS, F. J. A. Introduction to experimental phytochemistry. São Paulo, Brazil: UFC Editorial. 1997.

OLIVEIRA, VB et al. Effect of different extractive techniques on yield, antioxidant activity, total assays and clae-dad profile of dicksonia sellowiana (presl.). Hook, dicksoniaceae. Brazilian Journal of Medicinal Plants , v. 18, p. 230-239, 2016.

PADILHA, Itácio QM et al. Antimicrobial activity of Mimosa tenuiflora (Willd.) Poir. from Northeast Brazil against clinical isolates of Staphylococcus aureus. Revista Brasileira de Farmacognosia, v. 20, p. 45-47, 2010.

PEDROSO, Reginaldo dos Santos; ANDRADE, Géssica; PIRES, Regina Helena. Medicinal plants: an approach to rational and safe use. Physis: Revista de Saúde Coletiva, v. 31, 2021.

POZETTI, GL et al. Determination of antimicrobial activity of Brazilian plants. Magazine of the Faculty of Pharmacy and Dentistry, Araraquara , v. 6, p. 29-33, 1972.

RAMOS, Maria Isabel Lima et al. Nutritional quality of bocaiuva pulp Acrocomia aculeata (Jacq.) Lodd. Food Science and Technology, v. 28, p. 90-94, 2008.

RIBEIRO, C. V. C.; KAPLAN, M. A. C. Tendências evolutivas de famílias produtoras de cumarinas em angiospermae. Química Nova, v. 25, n. 4, p. 533–538, jul. 2002.

RODRIGUES, TS et al. Drying methods and yield of Plectranthus barbatus (Boldo da Terra) and P. ornatus (Boldo-Miúdo) leaf extracts. Brazilian Journal of Medicinal Plants , v. 13, p. 587-590, 2011.

ROESLER, Roberta et al. Antioxidant activity of cerrado fruits. Food Science and Technology, v. 27, p. 53-60, 2007.

SADIQ, Abdul et al. Antibacterial and antifungal potentials of the solvents extracts from Eryngium caeruleum, Notholirion thomsonianum and Allium consanguineum. BMC complementary and alternative medicine, v. 16, n. 1, p. 1-8, 2016.

SCORZONI, Liliana et al. Searching new antifungals: The use of in vitro and in vivo methods for evaluation of natural compounds. Journal of microbiological methods, v. 123, p. 68-78, 2016.

SILVA, DB et al. Vegetative propagation of Brosimum gaudichaudii Tréc. (mama-cadela) by root cuttings. Brazilian Journal of Medicinal Plants, v. 13, p. 151-156, 2011.

SILVA, Maria de L. et al. Bioactive oleanane, lupane and ursane triterpene acid derivatives. Molecules, v. 17, n. 10, p. 12197-12205, 2012.

SILVA, Nina Claudia BARBOZA et al. Use of medicinal plants in the quilombola community of Barra II-Bahia, Brazil. Latin American and Caribbean Bulletin of Medicinal and Aromatic Plants, v. 11, no. 5, p. 435-453, 2012.

SILVA, Wenderson Costa et al. Atividade in vitro de extratos e frações de Copernicia prunifera (Arecaceae) contra bactérias de importância clínica em saúde pública. Arquivos de Ciências da Saúde da UNIPAR, v. 27, n. 1, 2023.

SIMÕES, C. M. O., SCHENKEL, E. P., DE MELLO, J. C. P., MENTZ, L. A., & PETROVICK, P. R. Pharmacognosy: from natural product to medicine. Artmed Publisher. 2016.

SOARES, Sara Pimenta et al. Antibacterial activity of Stryphnodendron adstringens crude hydroalcoholic extract on dental caries microorganisms. Revista Odonto Ciência , v. 23, no. 2, 2008.

SOUSA ARAUJO, Thiago Antônio et al. Phytochemical, antioxidant and cytotoxic analysis of Brazilian Cerrado plants: Preliminary evidence of their antitumor activity. Journal of Medicinal Plants Research, v. 9, n. 9, p. 310-319, 2015.

UCHÔA, Valdiléia Teixeira et al. Free radical scavenging ability of Ximenia americana L. stem bark and leaf extracts. Journal of Applied Pharmaceutical Science, v. 6, n. 2, p. 091-096, 2016.

VENUGOPALA, Katharigatta Narayanaswamy; RASHMI, V.; ODHAV, Bharti. Review on natural coumarin lead compounds for their pharmacological activity. BioMed research international, v. 2013, 2013.

VIEIRA, Giciane C. et al. Warifteine, an alkaloid of Cissampelos sympodialis, modulates allergic profile in a chronic allergic rhinitis model. Revista Brasileira de Farmacognosia, v. 28, p. 50-56, 2018.

YANG, You; LAVAL, Stephane; YU, Biao. Chemical synthesis of saponins. Advances in carbohydrate chemistry and biochemistry, v. 71, p. 137-226, 2014.

ZHENG, L.; ZHAO, T.; SUN, L. Research progress of the pharmacological action and pharmacokinetics of coumarins. Shizhen Guoyi Guoyao, v. 24, n. 3, p. 714-7, 2013.

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Publicado

18-07-2023

Como Citar

DA SILVA, Antônia Fernanda Lopes; SIQUEIRA, Francisléia Falcão França Santos; DA SILVA, Wenderson Costa; NOBRE, Márcio Leonardo de Morais; PEREIRA, Fátima Maria de Souza; DA SILVA, Vitor Emanuel Sousa; DE OLIVEIRA, Francinete Sousa; LAGO, Eliana Campêlo. PHYTOCHEMICAL ANALYSIS AND ANTIMICROBIAL POTENTIAL OF EXTRACTS AND FRACTIONS OF BROSIMUM GAUDICHAUDII TRÉCUL AGAINST BACTERIA OF CLINICAL IMPORTANCE. Arquivos de Ciências da Saúde da UNIPAR, [S. l.], v. 27, n. 7, p. 3343–3363, 2023. DOI: 10.25110/arqsaude.v27i7.2023-007. Disponível em: https://unipar.openjournalsolutions.com.br/index.php/saude/article/view/10126. Acesso em: 21 dez. 2024.

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v. 27 n. 7 (2023)

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